Gene description for Hspa8
Gene name heat shock protein 8
Gene symbol Hspa8
Other names/aliases 2410008N15Rik
Hsc70
Hsc71
Hsc73
Hsp73
Hspa10
Species Mus musculus
 Experiment description of studies that identified Hspa8 in Breast cancer cells

1
Experiment ID737
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 25220352   
OrganismMus musculus
Experiment descriptionSurface functionalization of exosomes using click chemistry.
AuthorsSmyth T, Petrova K, Payton NM, Persaud I, Redzic JS, Graner MW, Smith-Jones P, Anchordoquy TJ.
Journal name Bioconjug Chem
Publication year2014
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.1-1.2 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140392: EV-METRIC:14%

2
Experiment ID1051
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name67NR
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

3
Experiment ID1052
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name66cl4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

4
Experiment ID1053
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

5
Experiment ID1054
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1.2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

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